recombinant protein  (R&D Systems)

Journal: Nature cell biology

Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells.

doi: 10.1038/s41556-025-01663-4

Figure Lengend Snippet: Fig. 5 | ITGB1 is a substrate for PTP-PEST and Shp2. a,b, A malachite green assay for free phosphate release after incubation of phosphorylated/non- phosphorylated ITGB1 peptides with recombinant Shp2 (n = 5 independent replicates, each performed in triplicate) (a) or PTP-PEST (n = 4 independent replicates, each performed in triplicate) (b). The significance was assessed using a Kruskal–Wallis test with a Dunn’s correction for multiple comparisons. The data are presented as the mean ± s.e.m. c,d, Schematics of FRET experiments (left) using mRuby2-tagged ITGB1 and Clover-tagged PTPs. Representative FLIM–FRET images (right) and quantification of apparent FRET efficiency of MM231 cells with stable expression of either ITGB1(WT)–mRuby2 or ITGB1(YYFF)–mRuby2

Article Snippet: Each fragment (2,400 pmol per peptide per reaction) was incubated separately for 1 h at 37 °C with recombinant Shp2 (0.05 μg ml−1; R&D Systems, 1894-SH-100) or PTP-PEST (0.05 μg ml−1; SignalChem, P39-21G-10) in phosphatase buffer (HEPES buffer, pH 7.5 (50 mM)/EDTA (0.2 mM)/DTT (5 mM)/Triton X-100 (0.01%)), before incubation with Malachite Green Reagent (100 μl per reaction).

Techniques: Malachite Green Assay, Incubation, Recombinant, Expressing

Journal: PLoS ONE

Article Title: Fine Specificity and Molecular Competition in SLAM Family Receptor Signalling

doi: 10.1371/journal.pone.0092184

Figure Lengend Snippet: Recombinant SAP, EAT-2, SHP-2 N+C and NK92 cell lysates were analysed by Western blotting using the LI-COR Odyssey Sa system. A representative blot for each protein is shown. 1×10 6 NK92 cells express 30 ng of SAP and 7 ng of EAT-2. 0.1×10 6 NK92 cells express 92 ng of SHP-2. One representative blot of each protein is shown.

Article Snippet: SHP-2 levels in 10 5 cell equivalents were analysed in a similar manner with recombinant SHP-2 N+C as a standard (25, 50 and 100 ng) and anti-SHP-2 (Cell Signaling #3752).

Techniques: Recombinant, Western Blot